Phytoplasma genomes contain large numbers of transposon genes and insertion sequences.
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The "'Insertion sequence IS1222 ribosomal frameshifting element "'is an transposition.
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The " S . haemolyticus " genome also contains many insertion sequences ( ISs ).
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This may be due to the structure of PAIs, with direct repeats, insertion sequences and association with tRNA that enables deletion and mobilization at higher frequencies.
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The repair template is designed for each application, as it must overlap with the sequences on either side of the cut and code for the insertion sequence.
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While at Stanford, Falkow encouraged Esther Lederberg to continue directing the Stanford Plasmid Reference Center, an internationally used registry for plasmids, transposons and insertion sequences .)
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Its gene occurs in c . 1-3 % of B . fragilis isolates, but fewer produce the enzyme since expression demands appropriate migration of an insertion sequence.
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Transposable element can be mainly classified as two classes : One class is very simple, called insertion sequence ( IS ), the other class is complicated, called transposon.
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Tn " 10 " has a composite structure and it is composed of a pair of insertion sequence elements ( IS " 10 " ) flanking five genes.
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As the aforementioned procedures are of particularly low efficiency, there is a need to identify the cells that have been successfully transfected with the vector construct containing the desired insertion sequence in the required orientation.
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